Seed is the most and basic part of the plant that plays the
role of re-growing the plant, conserving and transferring the
plants genetic material. It has also basic effect on dispersion,
propagation and plant viability in hard conditions. Seeds have
supported the great part of the food of the human, animal and
birds. In addition, the seeds have many medicinal, industrial
and commercial effects in human life. Human civilization was
started with seed recognition, collection, sowing and harvesting
the seeds.
Although in-situ conservation is the best for maintaining the
plant genetic resources, many difficulties such as climate changes,
irregular plant use, over grazing and also natural and unnatural
events pushed to ex-situ plant conservation. Especially this
method has grate importance in conserving the plants that are
in danger and extinction. Depending upon the seeds or plant
material resources, they are conserved in wide range of cold
temperatures that is between zero to -196 degree centigrade.
Documents have shown that natural resources gene-bank division
has begun its activities since 1960 decade in Research Institute
of Forest and Rangeland (RIFR). Because, the plant genetic resources
conservation have most priority, especially conserving the endemic
and also plants that are under extinction, RIFR decided to increase
the level of the gene bank unit to Gene bank division. So, it
restarts to reorganize the collection and evaluation of the
range, medicinal and forest plant genetic materials.
The organization chart of the natural resources gene bank, as
follow:
1- Seed collection and Identification Group (SIG)
2- Evaluation and Regeneration Group (ERG)
3- Protection and Conservation Group (PCG)
4- Information and Data Management Unit (IDMU)
Seed collection and Identification
Group (SIG)
This group includes 3 units;
Forest plants unit - Range plants unit- Medical plants unit.
The specialists of the seed collection and Identification group
with cooperation of 28 province experts are collecting the suitable
seeds according the standard protocol. It is determined to collect
8000 seeds for the range and medicinal plant accessions. After
seed collection, it is tried to identify the genus and species
name of the plant seeds.
Evaluation and Regeneration Group (ERG)
This group having 3 units:
-Genetic and Breeding unit-Biotechnology unit-Germplasm and
Seed Regeneration unit.
Cytogenetic laboratory, which is equipped with microscope having
monitor system and electrophoresis, conducts the cytogenetic
evaluation and seed morphological characteristics to prepare
the endemic seed color atlas. Cytogenetic laboratory tries to
determine the chromosome number of the collected seeds for clearing
the possibility of crossing between species.
Furthermore the mitotic, and meiotic divisions, DNA and protein
electrophoresis are being carried out in this laboratory. Cytogenetical
studies covered 21genus of legumenoseae family, including 65
Onobrychis species and one genus of grass family which included
25 Lolium species. Biotechnology laboratory has been equipped
with tissu culture chamber and instruments. Research activities
in biotechnology laboratory include:
-Propagation of the plants that their seeds no emerged or the
plants showing abnormalities
-Assaying the seed health via culturing the seeds on PDA
-Studying some parts of the Nitrogen fixation processes and
mycorrhiza also.
Field germplasms that are used for regeneration and Evaluation
of the seeds covered 7 hectare - in alborz research center.
So far (2004), 1700 accessions were regenerated or identified.
Regeneration covered the seeds, which have been reduced their
germination percentage or their quantity decreased by exchanging
processes.
Protection and Conservation Group (PCG)
This group has 5 units as follow:
• Seed Exchange Unit
• Seed Storage and Cold room Unit
• Cleaning unit and
• Seed Technology Unit
In seed exchange unit, after recording all of the seed requests,
if the requested seed were available in active cold room, it
would be handed over to applicant. Most of the applicants are
from universities, research institutes, research centers and
students of different colleges. Within last seven years, the
numbers of seed accessions in which presented to our colleagues
were more than 1950. In this group, after receiving the seed
accessions, there are given a new code number, cleaned with
particular methods and then hand over to seed technology unit
for different standard seed testing. After completion of mentioned
processes, each seed sample would be divided in two samples
and packed in aluminum and polyethylene packs. These samples
will be kept in permanent and active cold rooms respectively.
It is clear those less quantity seed samples, seed samples with
low germination percentage, decreased seed samples following
seed exchange would be in queue of regeneration. In seed technology
unit, all the standard seed testing, such as moisture percentage,
1000 seed weight, germination percentage and viability would
be carried out and recorded. In the case of facing with any
abnormalities, different needed seed treatments would be implemented.
Information and Data Management
Unit (IDMU)
Because of importance of seed information and data, in information
and data management unit, these are recorded and saved in particular
unit. After finishing the seed testing, all the recorded information
would be given to IDMU. In this unit, all the data would be
saved in such a way in which obtaining different characteristics
of each plant family, genus, species or accessions with their
distribution map would be possible.
So far, the number of accessions, which have been recorded,
reached more than 15000. Records have shown that seed samples
have been sent to applicants according their request .It includes
600 seed samples for provinces research centers, 250 for different
research divisions in RIFR, 400 seed sample for universities
and colleges and 700 seed samples for our colleagues in gene
bank. For being certain of safe keeping the seed information
and data, back up files have been prepared.